Custom Purification Services

Small Scale (<1g) and Pilot Purification Services up to 10-20g Scale

LSI scientists have a combined 70+ years of experience in custom purification of biomolecules. We offer a range of custom chromatography resin development and column packing services to support small, emerging biotechnology companies, primarily in the Boston area. Recent projects have centered on the purification of biological molecules, such as monoclonal antibodies (mAb), expressed proteins, nucleic acids, and glycoproteins. They are summarized in more detail below:

Landau Scientific Innovations offers a range of custom purification services centered on the production of biological molecules, such as monoclonal antibodies (mAb), expressed proteins, nucleic acids and glycoproteins. These services employ existing commercially available resins or may utilize custom affinity resins to support purification workflow development of small (<1 g) to pilot scale (10-50g) non GMP manufacture.  LSI also offers in-house training with several options available for entry level through advanced operator training.

Examples of Purification Services Available

  • Purification of antibodies via affinity ligand capture (rProtein A) followed by polishing by ion exchange (IEX) or mixed mode resins in small scale (<1 g) up to pilot (50g) scale batches;
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    • Purification from CHO cell culture sources,
    • Purification from serum or plasma sources,
    • Purification of antibody-drug conjugates.
  • Purification of expressed proteins, such as Green Fluorescent protein (GFP) in small scale (<1 g) up to pilot (50g) scale batches;
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    • Purification from bacterial cell expression systems,
    • Initial capture by IEX and mixed mode resins,
    • Final polishing to > 98% purity by large scale size exclusion chromatography (SEC)
  • Oligo dT, polynucleotides for capture of mRNA and viral particles,
  • Immobilized lectin affinity chromatography
  • Full analytical services support available for custom purification workflow;
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    • Antibody titer by HPLC Protein A affinity assay,
    • Affinity ligand leachate monitoring by ELISA assay,
    • dsDNA monitoring by qPCR,
    • SEC characterization of aggregation content of target molecule,
    • Full iso electric focus (IEF) characterization of charge variants of the target molecule or its impurities,
    • Full characterization by SDS-PAGE analysis,
    • Western blotting services also available,
    • Both in-geland on-blottrypsin digestion for micro-sequencing and peptide-based identification by LC-MS/MS,
    • Glycan analysis by LC-MS/MS

Contact us today to see how we can fill your biomolecule purification needs.